The theory of pH buffers was first outlined to meet the growing requirements in biomedical research for pH control. Growing mammalian cells in culture media is highly pH-sensitive; the addition of a pH buffer stabilizes pH and ensures successful cell growth.
Some pH buffers are almost transparent, having been produced to use in ultraviolet and invisible spectroscopy. They are highly suitable for spectrophotometric determinations due to their low pH range.
PH buffers are added before fermentation in the production of alcohol, m